General information
• Workplace: Paris (75%) and Saint-Malo (25%)
• Date of publication: July 2021
• Scientific supervisors : Pr Soizic Prado (MNHN) & Dr Cécile Le Guillard (CMI Roullier)
• Type of Contract: internship
• Period: 6 months
• Start date of internship: January 2022

Description of the internship topic
Context: this Master 2 internship will be carried out in the context of the SEABIOZ ANR project
involving both academic partners (Roscoff Biological Station, IRISA – Rennes, Natural History
Museum – Paris) and an industrial partner (Agro Innovation International, CMI Roullier – SaintMalo). This 4-year project, started in 2021, aims at providing new insights in the Ascophyllum
nodosum holobiont by evaluating the impact of season, origin, and microbial communities on
biological properties of seaweed extracts. A. nodosum is a common brown alga found along
the coasts of the North Atlantic Ocean. Very little is known so far about the importance of these
symbionts for the seaweed, except that they are believed to protect A. nodosum germlings
from desiccation and improve their robustness to different stresses. A. nodosum also
constitutes a prominent raw material used for many years to produce plant biostimulants and
solutions for the agriculture. Different bioactive molecules such as original polysaccharides
(laminarin, fucoidans, alginates), mannitol, betaines, polyamines, and polyphenolic
compounds, could be involved in these properties but the chemical nature and the
biosynthetical origins of the active molecules remain unknown.
Internship goals: The main objective of the internship will be to investigate the chemistry and
the biological activities of metabolites isolated from microbial strains associated with A.
nodosum. These isolated microbial strains will be cultivated in specific media appropriate for
fungi and bacteria development according to standard protocols developed by MNHN. The
microbial crude extracts will be produced following MNHN protocols to maximize the recovery
of a large number of metabolites and following the AII Roullier requirements which has a
restrictive list of solvent and cost constraints linked to the process upscaling. The analytical
annotation of those fungal and bacterial metabolites will be performed by several mass
spectrometry tools developed by MNHN and AII Roullier including metabolomic and molecular
networking. This approach will be combined with research in natural products and in-house
In vitro bioassays will be carried out at the CMI Roullier to determine the most active crude
extracts for biostimulant properties. Compounds will be thus specifically isolated from active
crude extracts obtained from large-scale cultivation of microorganisms. Pure compounds will
be obtained by different purification techniques (precipitation, ultrafiltration, preparative
chromotagraphy, SPE, …) and then fully characterized by Nuclear Magnetic Resonance (NMR)
and MS spectroscopies.

Student profile: The recruited Master 2 student will have strong experience working at the
microbiology / chemistry of natural substances interface and will have a strong interest in
fundamental research and in applied research.
• Chemistry: experience in chemistry of natural substances: Isolation and
characterization of molecules by MS, NMR Microbiology: knowledge or interest in
microbiology will be an asset.
• Biology: knowledge or interest in plant physiology/biology will be an asset
• Communication skills: english skills, writing of regular reports

Additional Information
Applications should include a detailed CV; at least 1 reference (persons we can contact); a onepage cover letter; notes/results of M1.

Pr. Soizic Prado (
Dr. Cécile Le Guillard (
Application deadline: 04/10/2021.

PhD position offer at National Museum of Natural History, France and Marine Station of Banyuls, Sorbonne University, in Chemistry & Microbiology


Type of contract: 3 years PhD
Start on October 1st, 2020
Application deadline: June 1st, 2020

Title: Bio-inspired isolation and identification of the ecological roles of compounds disrupting algal biofilms: an innovative approach for the identification of new eco-compatible antifoulings

Send CV, motivation letter and the name of two referees by email
to: Prof. Soizic Prado: and Dr. Raphaël Lami:

Profile of the student: The recruited doctoral student will have strong experience working at the microbiology / chemistry of natural substances interface involving:
1) Mastering the culture of microorganisms and molecular microbiology tools for genomic analyzes (metabarcoding, metagenomics, qPCR
2) Experience in chemistry of natural substances: characterization of molecules by MS, NMR and metabolomics.
3) Good interpersonal skills, ability to work in a team. Ability to write scientific publications and mastery of scientific English.

Description: Biofilms are complex communities of microorganisms in contact with a surface and included in a matrix that they secrete. Although beneficial in many environments, these nmicrobial biofilms are the source of many problems for military, civilian, industrial, and nrecreational marine activities. Thus, the fight against the development of biofilms is currently an important challenge in many industries, such as the production of underwater electricity, boat building, naval transport, desalination of seawater, and many others. The cost of induced corrosion thus represents between 3 to 4% of GDP in Western countries. These biofilms are also reservoirs of pathogenic organisms and resistant to antibiotics: the fight against their development constitutes in certain contexts a major health issue (food industry, hospitals).
Most of the used antifouling compounds are particularly toxic biocides, which are
applied in large quantities on the hulls of military and commercial ships, but also on
pipelines, port structures, power plants, and others. They have a significant impact on the natural environment because they are released into sea or river waters and accumulate throughout the food chain, leading to a high concentration of toxic substances in marine mammals and filter feeders. At present, no sustainable and environmentally friendly alternative has been developed on a large scale to solve this major environmental problem due to the lack of identified compounds with low ecotoxicity. However, these chemical compounds exist in Nature, and many marine species have developed strategies to protect themselves from biofouling. This is particularly the case of macroalgae (such as Delisea pulchra), which are capable of secreting molecules targeting quorum sensing, a mode of intercellular communication and involved in the formation of biofilms, thus limiting the colonization of pathogenic bacteria. An innovative strategy to find new sustainable, environmentally friendly natural compounds with antifouling activity is, therefore, to target the main mechanisms responsible for the formation of biofilms without affecting cell viability to select low toxic compounds. In this sense, the application of compounds with quorum quenching activity, capable of inhibiting quorum sensing, could meet these criteria and constitute a promising strategy against biological fouling. The knowledge of the algal holobiont has revealed the existence and major ecological roles of the microbiota associated with macroalgae capable of impacting the health of the host alga. Indeed, the algal epimicrobiota is the place of an intense and dynamic, multidirectional, and multi-partners chemical communication, which finely regulates the organization of surface biofilms of the alga. The key mechanisms involved in the formation of these biofilms have been little studied, but the co-supervisors of this project have identified some of them. Thus we have demonstrated that the fungal microbiota of the alga Saccharina
latissima, a key species of the northern European coasts, is the place of the production of
chemical mediators capable of inhibiting bacterial QS and by extension inhibiting the
formation of biofilms (Blanchet et al., 2017; Tourneroche et al., 2019). However, the precise identification of these compounds, as well as the precise understanding of their ecological role, has not yet been studied.
This multidisciplinary project combining Chemistry of Natural Products and Microbial
Ecology aims getting inspiration from the molecular dialogue of the micro-ecosystem hosted by the brown alga Saccharina latissima to find new sustainable natural compounds, respectfull of the environment, with antifouling activity and to depict the detailed molecular mechanisms involved.

The project is structured in 4 axes:

Axis 1: Microbial diversity of the algal microbiota of S. latissima. Epiphytic microbial diversity (bacteria and fungi) will be characterized by metabarcoding (16S rRNA and ITS) from sporophytes of S. latissima collected in collaboration with the Roscoff Biological Station. A culture-dependent approach will also be carried out to generate a collection of cultivablestrains for the isolation in sufficient quantity of the compounds of interest.

Axis 2: Fine chemical characterizations of the chemical mediators governing the QS of
biofilms of S. latissima. The biofilm metabolites will be extracted from the surface of S.
latissima and their chemical analysis will be carried out using a non-targeted and targeted metabolomics approach (search for specific molecules of QS) using mass spectrometry (MS) data. The metabolites will be annotated using the different databases available in our institutions. The extracts will be evaluated for their anti-QS and anti-biofilm activities according to protocols mastered by our labs. The correlation between microbial diversity (axis 1), metabolomics and QS activity will make it possible to link metabolic production to producing strains and to QS (meta-metabolomic) activity. The strains producing the chemical mediators of interest will be cultivated, alone or in combination, on a large scale, in order to isolate the compounds capable of interacting with QS specifically. The structural analysis of the compounds will be carried out by NMR and MS on the analytical platforms of the MNHN.

Axis 3. Characterization of the functional role of chemical mediators governing the QS of
biofilms of S. latissima. The description of the functional roles towards QS of the compounds characterized in axis 2 will be carried out by nanostring or RNAseq  approaches by specifically targeting the key genes involved in the metabolic pathways of QS and the formation of biofilms (eg luxI, luxM, luxR). A transcriptomic approach will be carried out on the epiphytic community in the presence of the compounds identified in Axis 2 in order to characterize the biosynthetic pathways potentially suppressed / activated within the community and to better understand the ecological role of the identified compounds.

Axis 4. Environmental impact of the characterized compounds, translational aspects scientific communication and general public. The environmental impact of the characterized compounds will be tested on target organisms in the aquatic environment (e.g. daphnids, Medaka fish). This aspect will be fundamental before considering the translational aspects.



PhD and postdoc positions at Wageningen University on computational genomics (Marnix Medema)

The vacancies (including more details on the content) can be found here:
For more information about this position, please contact our colleague Dr. Marnix Medema (tel. +31 317 480 944). For information on the selection procedure, please contact

Postdoc position in China: chemical ecology of seeds and seedlings (Xishuangbanna Tropical Botanical Garden, CAS)

Botanical garden

General information

Workplace : Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Yunnan Province, CHINA. The garden’s website ( provides a very complete overview of the facilities and the research and other activities carried out at XTBG.
Name of the responsible scientists : Doyle McKey (CEFE, Montpellier, and visiting professor at XTBG) and Jin Chen (Professor, director of XTBG)
Type of contract : CDD
Contract period : two years, can be prolonged
Start date : Ideally March 2020, can be discussed
Proportion of work : Full time
Remuneration : 15 000 RMB (Yuan) monthly (= 1937 € or $US 2180), coupled with very low living expenses (see details below)
Eligibility : Applicants should be younger than 35 years old, and should have obtained the PhD degree within the last three years.

Detail description of the project:

Post-doc Position description_XTBG_Chemical_ecology_seeds_and_seedlings

A joint workshop on fungal secondary metabolites, 30 Sept-2 Oct 2019, Hannover, Germany

This workshop is jointly co-organized by the National Museum of Natural History (S. Prado), the Westerdijk Fungal Biodiversity Institute (J. Collemare) and the Leibniz Universität Hannover (R. Cox). It will take place at the Leibniz Universität Hannover. Continue reading “A joint workshop on fungal secondary metabolites, 30 Sept-2 Oct 2019, Hannover, Germany”